12/28/2023 0 Comments Snapgene viewer pdest 17Thus, our system accelerates the production of functional proteins, which could be widely expressed in a lar ge variety of vertebrate organisms without tediously In vitro recombination of DNA with high speed, accuracy, and reliability compared with the traditional Systematic generation of this set of pCS2+ backbone-based Gateway destination vectors allows for Here, we generated a set of Gateway destination vectors, named as pGCS (plasmids of Gateway in pCS2+) vectors, which can be fused to a choice of frequently used amino- orĬarboxyl-terminal tags, including MYC, HA, FLAG, His, GST, as well as eGFP fluorescent epitope. To date, a suite of vectors with pCS2+ backbone applicable for Gateway cloning system As a multipurposeĮxpression vector, pCS2+ backbone-based expression plasmids are widely used for high-level expression of messenger RNAs (mRNAs) or proteins in mammalian/avian culture cells or Xenopus/zebrafishĮmbryos. However, a universal system that is compatible for multiple organisms was lacking. Gateway vectors have been extensively developed to facilitate gene cloning in numerous species Medical Center, 6001 Forest Park Road, Dallas, TX 75390, USA. Present address: Department of Pharmacology, Howard Hughes Medical Institute, University of Texas Southwestern Present address: Integrated Science Research Center, Peking University, Beijing 100871, China. Hubei Key Laboratory of Cell Homeostasis, College of Life Sciences, Wuhan University, Wuhan 430072, China,Īnd 2College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, China Acta Biochimica et Biophysica Sinica Advance Access published October 26, 2016Ĭonstruction of a series of pCS2+ backbonebased Gateway vectors for overexpressing
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